Determination of Clostridium perfringenes in liver of men and women cadaver after death as an indicator for postmortem interrval

Background: Most medical research that is used to better understand how post-mortem interventions are estimated requires an examination of the physico-chemical properties of decomposition and the effects that environmental factors have on the decomposition process. Microorganisms are unique in nature and occupy almost every habitat. These organisms, which reside in larger hosts, form complex populations of many species and change rapidly when hosts die and are known as spoilage microbial populations. Due to the anaerobic environment of the corpse, Clostridium spp. And its association with post-mortem spoilage were selected to investigate the possible relationship between this microorganism and the time elapsed since it was extracted from the liver. Methods and materials: Specimens were obtained with permission from the Forensic Medicine Organization and Ethics ID from University Ethics Committee No. 020/9898 from 34 human livers that had been destroyed in various ways such as murder, suicide and suspected death. After homogenization, the samples were cultured on blood agar and SPS medium. After initial tests including hot staining, catalase and oxidase, other diagnostic tests such as hemolysis, stormy fermentation, and nagler tests were performed. Finally, the relationship between death and the isolated bacteria was analyzed. Results: The results showed that 8 bacterial strains were isolated from which 2 isolates were Gram negative, 2 isolates were Gram positive but with Clostridium difficile and 4 strains belonged to Clostridium. Interestingly, in all four samples the time after death was more than 50 hours, and in the samples taken, the time after death was reported to be between 19 and 192 hours according to the information contained in forensic files. Conclusion: Thus, it can be concluded that the time between the presence of Clostridium strain in the liver and the time elapsed since the presence of Clostridium strain in the liver confirms the death of the body more than 50 hours.